Journal: Journal of Clinical Virology plus

Article Title: Evaluation of STANDARD TM M10 SARS-CoV-2 assay as a diagnostic tool for SARS-CoV-2 in nasopharyngeal or oropharyngeal swab samples

doi: 10.1016/j.jcvp.2022.100129

Figure Lengend Snippet: Spearman's rank correlation between ORF1ab Ct values from the STANDARD TM M10 SARS-CoV-2 assay and the reference method. The variables displayed a very strong positive correlation between the two variables (r= 0.954, p value < .001).

Article Snippet: The under evaluation STANDARD TM M10 SARS-CoV-2 assay is designed for use with the STANDARD TM M10 system (SD BIOSENSOR), which conducts sample preparation, nucleic acid extraction and amplification and detection of the target sequences, using the real-time RT-PCR as the test method.

Techniques:

Journal: Journal of Clinical Virology plus

Article Title: Evaluation of STANDARD TM M10 SARS-CoV-2 assay as a diagnostic tool for SARS-CoV-2 in nasopharyngeal or oropharyngeal swab samples

doi: 10.1016/j.jcvp.2022.100129

Figure Lengend Snippet: Ct values comparison between the STANDARD TM M10 SARS-CoV-2 assay and the reference method. (a) In samples with high viral load (Ct value <25), no differences between the two methods were detected. (b) In samples with moderate viral load (Ct value ≤25 and ≥30), the STANDARD TM M10 SARS-CoV-2 assay had a better performance than the reference method ( p value= .001408). (c) In samples with low viral load (Ct value >30), the STANDARD TM M10 SARS-CoV-2 assay had also a better performance than the reference method ( p value < .001).

Article Snippet: The under evaluation STANDARD TM M10 SARS-CoV-2 assay is designed for use with the STANDARD TM M10 system (SD BIOSENSOR), which conducts sample preparation, nucleic acid extraction and amplification and detection of the target sequences, using the real-time RT-PCR as the test method.

Techniques:

Journal: HardwareX

Article Title: BioSamplr: An open source, low cost automated sampling system for bioreactors

doi: 10.1016/j.ohx.2021.e00177

Figure Lengend Snippet: A comparison of bioreactor autosamplers.

Article Snippet: A few commercial autosampling systems available for lab bioreactors include the BioProbe™ from Bbi-Biotech, the Omnicoll Fraction Collector and Sampler from Lambda Instruments, the Segflow™ by Flownamics, and the more general reactor sampling system, the EasySampler™ by Mettler Toledo.

Techniques: Filtration, Sampling

Journal: International Journal of Molecular Sciences

Article Title: Antisense Oligonucleotide in LNA-Gapmer Design Targeting TGFBR2—A Key Single Gene Target for Safe and Effective Inhibition of TGFβ Signaling

doi: 10.3390/ijms21061952

Figure Lengend Snippet: Cellular uptake, mRNA and protein kinetics of lead candidate X07091 = NVP-13. ( A and B ) Cellular uptake kinetics of A549 ( A ) and ReNcell CX ® ( B ) cells after 0–72 h of incubation with 10 µM X07091 = NVP-13 as measured with a specific bioprobe ( n = 3). ( C and D ) TGFBR2 mRNA kinetics in A549 ( C ) and ReNcell CX ® ( D ) established in a qRT-PCR over 6 d after a single treatment with 10 µM X07091 = NVP-13 ( n = 3). TGFBR2 mRNA levels were normalized to the housekeeper Gnb2l and normalized to untreated cells. ( E and F ) Densitometric analysis of TGFBR2 protein levels (Western blot) in A549 ( E ) and ReNcell CX ® ( F ) after 1, 4, 8, and 12 d of treatment with 10 µM X07091 = NVP-13 (n = 4 for A549 and n = 7 for ReNcell CX ® ). ( G and H ) Western Blot (WB) against TGFBR2 (Biorybt) in A549 ( G ) and ReNcell CX ® ( H ). For qRT-PCR and WB, all values were normalized to untreated controls. Statistics was calculated by Ordinary-one-way-Anova followed by Dunnett’s multiple comparison test. * p ≤ 0.05, ** p ≤ 0.01, ± = SEM.

Article Snippet: After harvest, the supernatant was collected, cells were counted and both, cell culture media supernatant and cell pellets, were assessed for their X07091 = NVP-13 content at Axolabs GmbH (Kulmbach, Germany) using a selective and sensitive bioprobe.

Techniques: Incubation, Quantitative RT-PCR, Western Blot, Comparison

Journal: International Journal of Molecular Sciences

Article Title: Antisense Oligonucleotide in LNA-Gapmer Design Targeting TGFBR2—A Key Single Gene Target for Safe and Effective Inhibition of TGFβ Signaling

doi: 10.3390/ijms21061952

Figure Lengend Snippet: In-use stability of lead candidate X07091 = NVP-13. ( A ) Integrity of NVP-13 in 0.9 % NaCl (saline) when incubated with different temperature conditions and for different time intervals. For incubation at −20 °C +/− 5 °C a denaturizing Ion-Pair-Reversed-Pair High Performance Liquid Chromatography (IP-RP-HPLC) with Electrospray-Ionization (ESI)/Mass Spectrometry (MS) was used for determination of relative purity and identity of NVP-13. Aliquots incubated at 5 °C +/− 3 °C were analyzed using an IP-RP-HPLC combined with UV/Mass spectrometry and samples incubated at 37 °C (37 °C as adjusted by the integrated thermostat of New Brunswick Galaxy 170 S Incubator, Eppendorf) were analyzed using IP-RP-UPLC with UV/ESI/MS. Results showed that NVP-13 content was stable under all tested conditions. Discrepancy for content and purity/integrity were within method variability. Values are given as mean and SEM. Determination of intact NVP-13 with a specific bioprobe in the supernatant of cell culture media supernatant of ReNcell CX ® ( B ) and A549 cells ( C ) after incubation with 10 µM NVP-13 for up to 72 h, n = 3. Line of best fit for ( B ) and ( C ) was calculated by a nonlinear fit function.

Article Snippet: After harvest, the supernatant was collected, cells were counted and both, cell culture media supernatant and cell pellets, were assessed for their X07091 = NVP-13 content at Axolabs GmbH (Kulmbach, Germany) using a selective and sensitive bioprobe.

Techniques: Saline, Incubation, High Performance Liquid Chromatography, Mass Spectrometry, Cell Culture